salivette cortisol instructions
The Salivette is a specialized device for collecting saliva samples to measure cortisol levels‚ designed for non-invasive and stress-free testing. Proper usage ensures accurate results.
Overview of Salivette Cortisol Collection
The Salivette is a cotton-based device designed for collecting saliva samples to measure cortisol levels. It consists of a tube with a swab attached‚ which absorbs saliva when placed in the mouth. This method is non-invasive and stress-free‚ making it ideal for repeated or at-home testing. The device is specifically designed to ensure accurate cortisol measurement‚ with a unique blue cap distinguishing it for cortisol testing. Samples are typically collected at specific times‚ such as morning and evening‚ to assess circadian cortisol patterns. The Salivette is easy to use‚ requiring minimal preparation‚ and ensures high-quality samples for laboratory analysis. Proper handling and storage are crucial for reliable results.
Importance of Proper Salivette Usage
Proper use of the Salivette is crucial for obtaining accurate cortisol measurements. Contamination or improper handling can lead to inaccurate results‚ affecting diagnosis and treatment decisions. The swab must not be touched with fingers‚ as oils from the skin can interfere with cortisol levels. Timing is also critical‚ as cortisol levels naturally fluctuate throughout the day. Collecting samples at specified times ensures reliable data. Additionally‚ avoiding food‚ drink‚ and oral medications for at least 30 minutes before collection prevents interference. Adherence to instructions minimizes errors and ensures high-quality samples for laboratory analysis. Proper usage also maintains patient safety and the integrity of the testing process.
Preparing for Salivette Cortisol Collection
Preparation involves avoiding food‚ drink‚ and oral medications for 30 minutes before collection. Ensure proper hygiene and timing to guarantee accurate cortisol measurement and reliable results.
Pre-Collection Preparations
Before collecting a saliva sample using the Salivette‚ ensure proper preparation to avoid contamination and ensure accurate results. Avoid eating‚ drinking‚ or taking oral medications for at least 30 minutes prior to collection. Additionally‚ refrain from brushing teeth‚ smoking‚ or any activities that may cause bleeding gums for 60 minutes before sampling. For cortisol testing‚ evening collections are often recommended‚ especially for screening conditions like Cushing’s syndrome. Rinse your mouth with water 10 minutes before sampling to remove any residual substances. Ensure the Salivette device is ready and follow the provided instructions carefully to maintain sterility and accuracy. Proper preparation is crucial for reliable cortisol measurement.
Timing Considerations for Sample Collection
Timing is critical when collecting saliva samples for cortisol measurement. Cortisol levels naturally fluctuate throughout the day‚ peaking in the morning and decreasing at night. For accurate results‚ samples should be collected at specific times as directed by healthcare providers. Morning collections are typically done immediately upon waking‚ before brushing teeth or eating. Evening samples are often collected late at night to assess basal cortisol levels. Avoid collecting samples within 30 minutes of eating‚ drinking‚ or taking oral medications. Consistent timing is essential for reliable cortisol measurement‚ especially in studies monitoring circadian rhythms or diagnosing conditions like Cushing’s syndrome. Adhere strictly to the recommended schedule for optimal accuracy.
Step-by-Step Salivette Cortisol Collection Process
Open the Salivette tube‚ remove the swab‚ and place it in your mouth for 45 seconds to absorb saliva. Replace the swab and secure the tube tightly.
Opening the Salivette Tube
To begin the collection process‚ carefully remove the protective cap from the Salivette tube. Ensure the swab remains attached to the suspension in the tube. Avoid touching the swab with your fingers to prevent contamination. Gently twist or pull the cap to expose the swab inside. Do not fully remove the swab from the tube until you are ready to place it in your mouth. This step is crucial to maintain sterility and ensure accurate cortisol measurement. Once opened‚ proceed immediately to the next step to avoid any potential issues with sample integrity. Proper handling at this stage is essential for reliable test results.
Placing the Swab in the Mouth
Gently place the swab inside your mouth‚ typically between your cheek and gum‚ ensuring it absorbs saliva effectively. Avoid chewing or biting the swab‚ as this may damage it. Let it remain undisturbed for approximately 2-3 minutes or until it is sufficiently moistened with saliva. During this time‚ you can keep your mouth closed to facilitate absorption. It’s important not to move the swab around excessively‚ as this could disrupt the collection process. Once the swab has absorbed enough saliva‚ carefully remove it from your mouth and proceed to the next step. Proper placement ensures a high-quality sample for accurate cortisol testing.
Waiting for Sufficient Saliva Absorption
Allow the swab to remain in your mouth for approximately 2-3 minutes‚ or until it appears visibly moistened or swollen with saliva. During this time‚ keep your mouth closed to prevent saliva from dripping out. Avoid chewing or moving the swab excessively‚ as this could damage it or reduce absorption efficiency. You may feel a slight tingling sensation as saliva is absorbed‚ but this is normal. Once the swab has absorbed enough saliva‚ it should look saturated or slightly swollen. Proper absorption ensures an adequate sample for cortisol analysis. Do not remove the swab until it has reached this state to guarantee accurate test results.
Post-Collection Handling of Salivette
Replace the stopper securely‚ label the tube with name‚ date‚ and time‚ and store it upright in a cool‚ dry place until shipping or analysis.
Replacing the Stopper
After collecting the saliva sample‚ carefully replace the stopper into the Salivette tube. Ensure it is tightly closed by listening for a distinct click to confirm it is secure. This step prevents contamination and leakage during storage or transportation. Properly replacing the stopper is crucial for maintaining sample integrity and ensuring accurate test results. Always handle the stopper by the rim to avoid touching the swab or the inside of the tube. If the stopper does not click into place‚ check for any obstructions and try again. A securely closed tube is essential for reliable cortisol analysis.
Labeling the Salivette Tube
Properly label the Salivette tube immediately after collection. Use a permanent marker to clearly write your name‚ the date‚ time of collection‚ and your physician’s name on the tube. Ensure all information is legible and complete‚ as this ensures accurate identification and processing of the sample. The tube may have a pre-printed area for labeling‚ so follow the provided template. Avoid using abbreviations or unclear handwriting. Double-check the information for accuracy before finalizing. Proper labeling is critical for maintaining sample integrity and ensuring the test results are correctly associated with your records. This step is essential for accurate cortisol analysis and timely reporting.
Storage and Shipping Instructions
After collection‚ store the labeled Salivette tube in a cool‚ dry place‚ away from direct sunlight. If testing is delayed‚ refrigerate the sample at 2–8°C to preserve cortisol levels. For shipping‚ place the tube in a sealed plastic bag with absorbent material to prevent leakage; Include ice packs in the shipping container to maintain the recommended temperature during transit. Ensure the package is clearly marked as a biological specimen and handled according to local regulations. Avoid exposing the sample to extreme temperatures or prolonged heat‚ as this may degrade cortisol levels. Promptly ship the sample to the designated laboratory to ensure timely analysis. Proper storage and shipping are critical for maintaining sample integrity and obtaining accurate cortisol test results.
Special Considerations for Salivette Use
- Avoid eating‚ drinking‚ or brushing teeth for 60 minutes before collection.
- Exclusion of alcohol 24 hours prior to sample collection is recommended.
- The device is not intended for children under three years old.
- Store samples away from heat sources to preserve cortisol levels.
Contraindications for Salivette Use
The Salivette is not suitable for children under three years old. Individuals with active oral infections or bleeding gums should avoid using the device. Patients must refrain from eating‚ drinking‚ or brushing teeth for at least 60 minutes before collection. Alcohol consumption should be avoided for 24 hours prior to sample collection. Additionally‚ the device should not be used by individuals with severe dental work or mouth injuries that could interfere with saliva collection. Proper handling is essential to avoid contamination‚ ensuring accurate cortisol measurement. Always store the Salivette in a cool‚ dry place to maintain sample integrity. Adhering to these guidelines ensures reliable test results.
Device-Specific Features for Cortisol Testing
The Salivette is designed with a unique blue screw cap for cortisol testing‚ ensuring easy identification. It features a cotton swab that absorbs saliva efficiently without causing discomfort. The device is sterile and individually wrapped to prevent contamination. After collection‚ the swab is securely placed back into the tube‚ which is then sealed tightly. This design ensures optimal cortisol extraction‚ with recovery rates consistently over 90%. The Salivette is compatible with various analytical methods‚ including LC-MS-MS‚ making it a reliable choice for accurate cortisol measurement. Its user-friendly design minimizes errors‚ and the provided biohazard bag ensures safe storage and transport of samples to the laboratory.
